Preparation of gelatin hydrogels incorporating small interfering RNA for the controlled release
نویسندگان
چکیده
منابع مشابه
Preparation of gelatin hydrogels incorporating small interfering RNA for the controlled release.
The objective of this study is to design biodegradable hydrogels of gelatin for the controlled release of bioactive small interfering RNA (siRNA). Gelatin was cationized by chemically introducing ethylene diamine into the carboxyl groups in different conditions to obtain cationized gelatins (CGs). The CG was mixed with siRNA at various mixing ratios of amino groups of gelatin to the phosphate g...
متن کاملSmall interfering RNA; principles, applications and challenges--
Gene silencing using RNAi (RNA interference), has recently been used as a successful laboratory technique in determining the function and control of gene expression and provides a wide range of applications in molecular biology and gene therapy. RNAi is a method of suppressing gene expression. In this direction, a single-stranded RNA molecule of about 21–23 nucleotides, called siRNA (small inte...
متن کاملGelatin nanospheres incorporating siRNA for controlled intracellular release.
The objective of this study is to develop a sustained release system of small interfering RNA (siRNA) inside cells aiming at a prolonged time period of gene suppression. Gelatin aqueous solution containing luciferase siRNA was coacelvated by acetone addition, followed by the glutaraldehyde (GA) crosslinking of gelatin to prepare gelatin nanospheres incorporating siRNA. The nanospheres were degr...
متن کاملPreparation of gelatin hydrogels incorporating low-molecular-weight heparin for anti-fibrotic therapy.
The objective of this study is to design biodegradable hydrogels for the controlled release of low-molecular-weight heparin (LMWH) and evaluate the biological activity. Gelatin was cationized by chemically introducing ethylene diamine into the carboxyl groups in different conditions to obtain cationized gelatins. The cationized gelatin was mixed with the LMWH in aqueous solution to form the com...
متن کاملAntimicrobial Modified-Tragacanth Gum/Acrylic Acid Hydrogels for the Controlled Release of Quercetin
In this study, new antimicrobial hydrogels were prepared via reaction of functionalized-tragacanthgum (TG) biopolymer by quaternary ammonium functionalization of TG (QTG) with acrylic acid(AA). Characterization of the QTG hydrogels (QTG-AA) was carried out by FTIR,thermogravimetric analysis (TGA), and 1H NMR. Dynamic mechanical analysis, (DMA) wasconducted to characteriz...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Drug Targeting
سال: 2012
ISSN: 1061-186X,1029-2330
DOI: 10.3109/1061186x.2012.725170